The Effect of Ionizing Radiation on Multi-drug Resistant Pseudomonas aeruginosa Isolated from Aquatic Environments in Egypt.

Aims: This study was conducted to determine the effect of different doses of gamma radiation on Multi-drug resistant Pseudomonas aeruginosa isolated from River Nile at Rosetta branch and associated drains in Egypt. Place and Duration of Study: The study was started with samples collection in August 2010 through April 2011 in the Microbiology Dep., Central Laboratory for Environmental Quality Monitoring (CLEQM), National Water Research Center (NWRC), Cairo, Egypt and the National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt. Methodology: Water samples were processed using membrane filtration, 144 strains of P. aeruginosa were isolated and identified and their antibiotic susceptibility was determined against 20 different antibiotics using agar disc diffusion method. Irradiation of bacterial isolates was processed using gamma irradiation unit of cobalt (Co60) and the D10-value was calculated from the survival curve. Results: Isolates were categorized as multi-drug resistant Pseudomonas aeruginosa (MDRPA). 125 (86.8%) were found to be extensively drug resistant (XDR) and 19 (13.2 %) were characterized as possible pan drug resistant (PDR). The highest resistance (100%) was mostly directed to amoxycillin/clavulanic acid, ampicillin, carbenicillin, methicillin, cephalothin, kanamycin, vancomycin, tetracycline, erythromycin, clindamycin, trimethoprim/sulfamethoxazole, nitrofurantoin and chloramphenicol. More than 75% of isolates were sensitive to norfloxacin (82.6%), piperacillin (81.2%), amikacin (79.2%) and tobramycin (77.8%). 63.2%, 26.4% and 14.6% of isolates were sensitive to ofloxacin, cefotaxime and ceftriaxone, respectively. The viable counts of MDRPA decreased with increasing radiation doses of gamma rays up to the lethal dose (3 kGy). The counts of 0.5, 1.0, 1.5, 2.0, 2.5 kGy irradiated samples were respectively 7.8, 6.5, 4.7, 2.3& 1 log10 and the D10-value calculated from the survival curve was 0.27 kGy. Conclusion: Contaminated fresh water may act as reservoirs for antibiotic resistant pathogens. Regular monitoring of Multi-drug resistant pathogens in aquatic environments should be adopted constantly. Gamma radiation demonstrates a potential value for wastewater treatment and pollution control.

Isolation of lipase producing fungi from palm oil Mill effluent (POME) dump sites at Nsukka

In this study, twelve fungal lipase producing strains belonging to Aspergillus, Penicillium, Trichoderma and Mucor genera were isolated from palm oil mill effluent composts. The Aspergillus spp. were more frequent (42 percent) and was present in all the samples assayed. Mucor sp. was the least encountered (8.3 percent).The lipase producing profile showed that Trichoderma (8.07-8.24 u/mL) and Aspergillus (6.25 -7.54 u/mL) spp. were the highest lipase producers while Mucor (5.72 u/mL) was the least.

Study on Current Status of Garbage and Waste Water Treatment in Rural Areas of Fujian and the Countermeasures / 环境与健康杂志

Objective To understand the current status of overall management and treatment of garbage and waste water in rural areas of Fujian,and to provide scientific evidence for governments at all levels to make policies in rural sanitation. Methods From September to December,2006,215 villages in 21 counties in Fujian rural areas were selected,and 10 families were sampled and investigated for garbage collection and waste water treatment from each village. Results In the investigated villages,the production of garbage amounted to 40 837.4 tons per month;and domestic and productive garbage accounted for 32.2% and 67.8% of the total,respectively. Among the domestic garbage,23.7% was randomly discharged or stacked,and only 8.5% went through harmless treatment (incineration,composting under high temperature or direct reuse). However,30.7% productive garbage was randomly discharged or stacked,and 37.1% went through harmless treatment. Every month,948 195 tons of waste water was produced in the investigated villages;57.5% of them were domestic and 42.5% were productive. Only 0.6% of the domestic waste water and 33.0% of the productive waste water were treated. Conclusion The rural public health infrastructure building is far lagged,with low rate of harmless treatment of garbage and waste water. Random littering and piling (discharging) garbage are common phenomena in rural areas. Thus,it is urgent to improve rural sanitation development.

Application of resonance charging circuit to electro-hydraulic impulse apparatus for waste water treatment / 医疗卫生装备

The application of resonance charging circuit to electro-hydraulic impulse apparatus for treating wastewater is introduced in this paper.The circuit permits the graduating increase of current without influence from time.The unique change of voltage on capacitor is favorable for the recovery of electric strength of electro-hydraulic impulse apparatus.The theory and design method of resonance charging circuit are provided.

Study on the removal of cresol for the agricultural waste water treatment / 日本農村医学会雑誌

Concerning the agricultural waste water treatment, adsorption removal of cresol which is one of disinfectants by commercial activated carbon was investigated.<BR>It was not found the significant correlation between amount of cresol adsorbed and physical properties of eight kinds of activated carbon. The intraparticle diffusivity of cresol onto the pores of activated carbon was significantly related to the mesopore volume.<BR>In the treatment of agricultural waste water, it is concluded that the activated carbon which is rich in mesopore volume is suitable for the adsorption removal of cresol from the standpoint of adsorption rate.

Study on the phenol removal for the security of water quality in rural areas / 日本農村医学会雑誌

For the security of water quality in rural areas, adsorption removal of phenol which is one of disinfectants for excretions by six kinds of commercial activated carbon was investigated.<BR>At equilibrium concentration of 1 and 10 ppm, activated carbon indicated pH 10.19 adsorbed phenol as much as 2.17 and 1.84 times compared with activated carbon indicated pH 3.06, respectively. At low equilibrium concentration of phenol, the amount adsorbed was significantly influenced by surface pH of activated carbon (p <0.05).<BR>These results suggest that the difference in amount of phenol adsorbed onto activated carbon is due to the interaction between phenol and surface oxygen groups on the adsorbent. Therefore, it is concluded that the activated carbon of which surface is basic suitable for the adsorption removal of phenol in the agricultural waste waters.